Polyphenol Testing & Scientific Verification
At Dalmatia Oil, we treat polyphenol analysis with the same seriousness as cultivation and production. Our published values are not marketing estimates — they are laboratory-verified measurements obtained using recognised scientific methodology.
All analytical work is conducted independently at the Department of Food Technology and Nutrition, University of Split.
Total Polyphenol Content
Total phenolic concentration is determined using the internationally accepted Folin–Ciocalteu method. This is a standard laboratory procedure used globally in food science to quantify overall phenolic content and antioxidant capacity.
Results are expressed in mg/kg and provide a reliable measurement of the oil's total phenolic strength.
Individual Polyphenol Profile
Specific phenolic compounds — including:
- Hydroxytyrosol — A powerful antioxidant linked to cardiovascular protection and cellular health.
- Tyrosol — Supports heart health and helps protect against oxidative stress.
- Oleocanthal — Known for its natural anti-inflammatory properties, similar to ibuprofen.
- Oleacein — Contributes to neuroprotection and anti-inflammatory benefits.
are analysed using UPLC (Ultra-Performance Liquid Chromatography) coupled with MS (Mass Spectrometry) detection.
This is an advanced, high-resolution analytical technique used in serious scientific research. It:
- Separates individual phenolic compounds with high precision
- Identifies each compound using mass detection
- Quantifies them against certified reference standards
- Requires specialised columns, solvents, and calibration materials
UPLC–MS is significantly more sophisticated — and more costly — than basic total phenol testing. It allows precise identification and measurement of individual bioactive molecules within the oil.
Our Commitment to Scientific Integrity
We combine:
- Recognised total phenolic testing (Folin–Ciocalteu)
- Advanced compound-specific analysis (UPLC–MS)
- Independent university laboratory verification
This ensures that every figure we publish is measurable, defensible, and scientifically robust.
When we describe our olive oil as high in polyphenols, it is supported by rigorous analytical evidence at university level — not marketing language.
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